mer.

27

janv.

2016

News from the cold!  Pasteur Institute Flu laboratory and APcis make collaborative research with Materia Medica Holding

 

Results of preclinical investigation of new antiviral Anaferon for children

 

 

 

Upon the basis of our laboratory research at APcis, we have studied the antiviral efficacy of a novel drug, Anaferon for children (antibodies to interferon gamma in released-active form manufactured by OOO “NPF “MATERIA MEDICA HOLDING”, Russia) alone or in combination with standard antiviral drug (oseltamivir phosphate, F. Hoffmann–La Roche Ltd., Switzerland) in various in vivo experimental models of influenza infection in mice, since 2008. The efficacy was assessed not only against important pandemic strain of Influenza (А/H1N1 (California/07/2009v), but also against other influenza А strain, A/H3N8 (Equi2/Miami/1/63) that show a severe pathogenicity in rodents. As there is often a great variability of strains during seasonal Flu, drug candidates with a broad antiviral spectrum are scrutinized with great expectations.

 

            In vivo experiment in murine model of А/H1N1 (California/07/2009v) infection at the dose of 10 LD50 has shown that Anaferon for children (20ml/kg/day) was effective against the virus both alone and in combination with oseltamivir (10 mg/kg/day). More precisely, administration of Anaferon for children alone has led to a later disease onset (+48 hours) after viral nasal challenge and to a decrease in the mortality rate (-20%). Combined treatments with Oseltamivir phosphate and Anaferon for children has also decreased the mortality as compared to Oseltamivir only group.

 

The mice’s health measured by bodyweight variations suggests that mice treated with Anaferon for children alone or in combination with oseltamivir phosphate were less affected by the challenge and that surviving mice recovered sooner than placebo treated mice. Anaferon for children was as efficient as oseltamivir (10 mg/kg/day) in Balb/c mice, but the most prominent antiviral properties were shown for the combination of two drugs (including later disease onset and overall reduction in severity of the infection).

 

In the model of A/H3N8 (Equi2/Miami/1/63) infection at the dose of 5 LD50, Anaferon for children (0,4 ml/mouse/day) was used in preventative and curative oral dosing regimen and was more effective than oseltamivir at the dose of 4 mg/kg/day, providing statistically significant reduction of the death rate over time. Both Anaferon for children and oseltamivir phosphate treatments reduced the duration of the clinical signs of infection and enhanced animals survival when compared to placebo.

 

Therefore, the results of our experiments have shown that the efficacy of Anaferon for children was comparable to that of oseltamivir phosphate. Moreover, it was shown that the antiviral activity of oseltamivir phosphate could be improved by Anaferon for children.

 

A following study on possible synergism of action of Anaferon for children and oseltamivir phosphate has been conducted in 2015 in the Flu Laboratory of Pasteur Institute in collaboration with us.

 

Anaferon for children has shown a statistically significant decrease in viral copies number of pandemic H1N1 strain in the infected cell culture alone, and then was subsequently tested in 3 different volumes in combination with oseltamivir at minimal effective dose (20nM) against different Influenza H1N1 strains (A/Danmark/524/09 Sensitive H1N1p and A/Danmark/528/09 Resistant H1N1p). Anaferon for children has decreased viral multiplication in presence of oseltamivir which was estimated by qRT-PCR quantification of the specific protein.

 

As expected, resistant H1N1 strain showed a complete resistance to the neuraminidase inhibitor (Oseltamivir at 20 nM). The addition of Anaferon for children to the same dose of oseltamivir has led to a significant antiviral effect for both Oseltamivir sensitive and resistant strains, H1N1pdm09 sen (A/Danemark/524/09 sen) and H1N1pdm09 res (A/Danemark/528/09 res), respectively. When combined with oseltamivir at 20nM, Anaferon for children tended to delay viral multiplication by approximately 24 hours.

 

These results suggest that the efficacy of Anaferon for children may be mediated by viral synthesis delay or inhibition, or by stimulation of cell endogenous cytokine production and engaging intracellular antiviral mechanisms, although this inhibition in a single dose – even applied prior to the viral challenge - is mild and incomplete. Anaferon’s eventual antiviral efficacy is similar with different Influenza strains causing flu epidemics but it is observed in the presence of oseltamivir at 20nM, and thus is compatible to a standard prescription level of the reference antiviral drug. The efficacy of Anaferon for children is additive to that of oseltamivir and the eventual increase in antiviral efficacy seems to be independent of neuraminidase inhibition, which was assessed in MUNANA fluorescence test.

 

 

 

Therefore, Anaferon for children has shown some antiviral activity in several in vitro and in vivo models of Influenza A infection, including pandemic influenza A/H1N1 both alone and in combination with oseltamivir and could be recommended as an effective product for treatment and prevention of influenza A infections in countries where efficacy and safety in man  have been documented.

 

 



 

 

 

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mar.

09

juin

2015

New travel Inside the mouse: Univ Virginia discovers lymph vessels to the brain

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mar.

19

nov.

2013

Aspergillosis : resoiratory infection, birds as models


One of the recent publications we contributed to has been published

 

Thierry S, Durand B, Melloul E, Tafani JP, Wang DY, Deville M, Cordonnier N, Deville M, Chermette R, Guillot J, Arné P. Assessment of Aspergillus fumigatus pathogenicity in aerosol-challenged chickens (Gallus gallus) belonging to two lineages. CIMID. 2013 Mar 19. S0147-9571(13)00005-2

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lun.

22

juil.

2013

Coronavirus strike back...

New Human Coronavirus may come from bats, although it is unlikely that a direct contact was pre-existing in the arabic peninsula where the outbreak of 2013 originated. Eventual link with genetic code of poultry and dogs and cats coronavirus that cause respiratory diseases (infectious bronchitis and collectivity rhinitis respectively), may thus be investigated further.

Initial sera analysis suggest that camels have developed antibodies against the New Human Coronavirus...

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lun.

15

juil.

2013

High resolution imaging: osteology in horse with cone beam

3D imaging of horse anterior with high resolution by cone beam scanning.

Analyse tridimensionnelle du membre antérieur du cheval.

 

 Introduction of an anterior of a horse in the largest cone beam scanner is a challenge: thanks to Pr Degueurce preparation, we managed to obtain 50 µm resolution throughout the foot carpian and metacarpian areas.

 

Au scanner Cone beam, la résolution est de 50 microns et le membre confié par le Pr Degueurce a été analysé finement de manière à permettre le développement d'un nouvel outil d'analyse.

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ven.

31

mai

2013

Predictability of Flu animal models in man: EMA update

The starting assumption for the use of animal models is that, if immunogenicity follows similar patterns in animal models and humans, it may be reasonable to extrapolate efficacy as well. The animal models used to study influenza include ferrets and mice, much less frequently guinea-pigs and non-human primates. The assessment of vaccine efficacy in ferrets includes challenge with high doses (105 ID50 or a lethal dose) of homologous and heterologous pandemic viruses followed by measurements of clinical signs and viral replication (serial nasal washes).

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lun.

08

avril

2013

Of mice and men...

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mar.

05

févr.

2013

Premiering : high resolution osteology with pregnant Swiss mouse and cone beam scanner

For the first time, fetal bones are clearly visible and the mouse gave birth to a healthy litter of 12 sucklings 4 days later.

 

Image was obtained on 128 Siemens CT scan, in 1/4 of a second, courtesy of Drs JF Paul (Marie Lannelongue Radiology unit) and C Vidal (Pasteur Institute) with Useful Progress software.

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mar.

05

févr.

2013

L'idée du corps

2012, L'idée du corps, une exposition entre art et sciences biologiques et médicales.

 

Après le corps dans tous ses états, AP6 a réuni une centaine d'artistes contemporains de Yoko Ono ou Barbara Hammer et Fluxus à Michel Journiac pour célébrer les momies du XVIIème siècle dont Fragonard et son musée d'Alfort.

 

L’idée du corps

 

 

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ven.

11

janv.

2013

Sauvons l'âne du Poitou!

Dépôt Génétique

Comme tous les pères et des femelles de race sont inscrits dans le Livre d'étude d'Âne Poitou, l'association a financé des programmes de recherche pour permettre le gel - le dégel de semence. 

Basé sur l'obtention fructueuse d'un diluant adéquat à l'École Vétérinaire de Nantes - le Laboratoire de Sciences de la Reproduction - en 1997, l'association crée maintenant un dépôt génétique dans le coeur même du pays d'originede la race. 

Insémination artificielle / transfert d'embryon

Le premier succès de transfert d'embryon et de la conservation de qualité de sperme suggère que les femelles puissent dans le monde entier profiter de la recherche sur la génétique des pères et contribuer ainsi pour sauver l'âne Poitou. 

Sauvez les Âne du Poitou, documents clés:

Où voir des Ânes Poitou vivants ? 
- L'événement suivant montrant des ânes du  Poitou :Salon de l'agriculture, Paris, mars 2014
- De manière permanente : Parc inter-régional du Marais Poitevin, F-171709 RONDE LA 

Comment obtenir des informations supplémentaires ?

 

Association pour la Sauvegarde du Baudet du Poitou - SABAUD 
President : Jacques MOREAU, DVM 
Directeurr : Benoît FOUCHIER 
Adresse : 600, avenue de Niort - F-79360 BEAUVOIR SUR NIORT
Tel : 33 (0) 549 09 72 39
Fax : 33 (0) 549 09 72 39 

 
HARAS NATIONAUX DE SAINTES 
Directeur : Mr DEVEVEY 
Adresse : avenue de Jourdan - BP 305 - 17107 SAINTES
Tel : 33 (0) 546 74 35 91
Fax : 33 (0) 546 74 80 11

 
PARC NATUREL REGIONAL DU MARAIS POITEVIN 
President : Mr André DULAIT. 
Adresse : 17170 LA RONDE
Tel : 33 (0) 546 27 82 44
Fax: 33 (0) 546 27 83 45

 
ASSOCIATION DES ELEVEURS DES RACES EQUINE, MULASSIERE ET ASINE, BAUDET DU POITOU 
President : Mr FAIVRE 
Adresse : 127, rue de la République - 85200 FONTENAY LE COMTE
Tel : 33 (0) 251 69 21 95
Fax : 33 (0) 251 69 22 93

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Bonne nouvelle!

Notre SRC est agrée pour le Crédit d'Impôt Recherche. Depuis que le dispositif existe nos clients bénéficient d'un crédit d'impôt en France équivalent au moins à 50 % du montant des études qu'ils nous confient.

Our CRO,part of excellence cluster MEDICEN, allows our client to benefit in France from state incentive up to over 50 p.cent of study cost.